Rad sa konferencije: Antibiotska rezistencija enterokoka izolovanih iz Travničkog/Vlašićkog sira, BiH

Abstract: Antibiotic resistance poses a safety risk to public health, involving the spreading of antimicrobial resistance between animals, humans and the environment, also consequently along the food chain. Limited studies have considered the spread of antibiotic resistance due to wild enterococci isolated from traditional cheese. Enterococcus faecalis and Enterococcus faeciumare the most common isolated enterococci from food, they can be found in different food sources (cheeses, meat, olives and vegetables). A variety of traditional cheeses made from raw and pasteurized milk contained enterococci as an essential part of natural microflora. The purpose of this work is a preliminary examination of antibiotic resistance in enterococci isolated from traditionalTravnički/Vlašićkicheese. As a result of 16S rRNA sequence analysis, 14 of the 21 enterococci strains were identified as E. faecalis, 6 as E. faecium and 1 strain as E.durans. The 21 Enterococcus strains were tested for susceptibility to 9 different antimicrobial agents by agar dilution method. A total of 19Enterococcusstrains displayed resistance to low concentrations of aminoglycosides, streptomycin (STR), gentamycin (GEN) and kanamycin (KAN) were analyzed with MIC > 64μgmL−1, MIC > 64μgmL−1and MIC > 32μgmL−1, retrospectively. Only 2 Enterococcus strains were sensitive to above mentioned MIC of aminoglycosides. On the susceptibility/sensitivity of Enterococcusspecies to β-lactams, allEnterococcusstrains were sensitive to ampicillin (AMP) (MIC > 16μgmL−1). However, 3 of the 14 E.faecalis strains as well as 3 of the 6 E.faecium strains and an E.duransstrain showed susceptibility to MIC > 16μg mL−1of penicillin G (PEN G). A total of 12 Enterococcus strains (57%) were sensitive to MIC > 16μg mL−1of tetracycline (TET). All chees enterococci strains displayed sensitivity to very high MIC > 8μg mL−1of erythromycin (ERY) as well as chloramphenicol (CHL) (MIC > 4μg mL−1), those breakpoints were commonly used for clinical isolates. 50% of Enterococcus strains showed resistance to MIC > 32μgmL−1of vancomycin (VAN), even if vancomycin-resistant enterococci (VRE) are important opportunistic pathogens with limited therapeutic options.

Keywords: Antibiotic resistance; Wild enterococci; TraditionalTravnički/Vlašićki cheese

Konferencija: 10th Central European Congress on Food, Springer

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